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KMID : 0356920090560040419
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Korean Journal of Anesthesiology
2009 Volume.56 No. 4 p.419 ~ p.424
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Contralateral allodynia and central change in the chronic post-ischemic pain model rats
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Kwak Kyung-Hwa
Jung Kyung-Young
Choi Jy-Young
Ryu Tae-Ha
Yeo Jin-Seok
Park Sung-Sik
Lim Dong-Gun
Kim Si-Oh
Baek Woon-Ei
Hong Jung-Gil
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Abstract
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Background: Mirror-image allodynia is a mysterious phenomenon that occurs in association with many clinical pain syndromes including complex regional pain syndromes (CRPS). Underlying mechanisms for the development of such pain are still a matter of investigation. Several studies suggest that activation of the N-methyl-D-aspartate (NMDA) receptor is essential for central sensitization as a base for persistent pain. The aim is to assess whether alteration of NMDA receptor expression correlates with the contralateral allodynia in the chronic post-ischemia pain (CPIP) model rats representing CRPS-Type I.
Methods:: Application of a tight-fitting tourniquet for a period of 3 hours before reperfusion produced CPIP in male Sprague-Dawley rats. The mechanical paw withdrawal thresholds to von Frey stimuli (using a dynamic plantar aesthesiometer) were measured as pain indicators in ipsilateral and contralateral hindpaws. Phosphorylation of the NMDA receptor 1 subunit (pNR1), assessed with Western blot, was measured in the contralateral L4-6 spinal cord.
Results: Ipsilateral and contralateral mechanical allodynia is present at 4 hours after reperfusion, peaked at 3 days, and continued for 7 days after reperfusion. The relative density of pNR1 of CPIP rats significantly decreased in the contralateral L4-6 spinal cord compared to baseline value (P ＜ 0.05). There was significant correlation between paw withdrawal threshold and the relative density of pNR1 (ipsilateral; R2 = 0.75, P ＜ 0.01, contralateral; R2 = 0.60, P ＜ 0.01).
Conclusions: These data suggest that pNR1 is correlated to the contralateral mechanical allodynia in CPIP rats.
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KEYWORD
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Chronic post-ischemia pain, Complex regional pain syndrome-type I, NMDA receptor, Mirror-image allodynia
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